Molecular cloning, characterization and expression analysis of cathepsin A gene in Chinese mitten crab, Eriocheir sinensis.

Molecular cloning and characterization of three novel Hemocyanins from Chinese mitten crab, Eriocheir sinensis

Molecular Cloning and Gene Expression Analysis of the Leptin Receptor in the Chinese Mitten Crab Eriocheir sinensis

BACKGROUND Leptin is an adipocyte-derived hormone with multiple functions that regulates energy homeostasis and reproductive functions. Increased knowledge of leptin receptor function will enhance our understanding of the physiological roles of leptin in animals. METHODOLOGY/PRINCIPAL FINDINGS In the present study, a full-length leptin receptor (lepr) cDNA, consisting of 1,353 nucleotides, wa...

Effect of temperature stress on the survival of juvenile Chinese mitten crab (Eriocheir sinensis)

Eriocheir sinensis, which is an important aquaculture species, belongs to the class Crustacea. To discuss the temperature tolerance of E. sinensis, the survival rates of juvenile crabs of E. sinensis were determined in this paper using a series of temperature settings (normal temperature, 25°C, 30°C, 32°C, 34°C, 35°C, 36°C, 38°C, 40 °C). The results indicated that except the normal temperature ...

Molecular cloning, characterization and expression analysis of macrophage migration inhibitory protein (MIF) in Chinese mitten crab, Eriocheir sinensis.

Macrophage migration inhibitory factor (MIF) as a multi-functional cytokine mediating both innate and adaptive immune responses, however, their function within the innate immune system of invertebrates remains largely unknown. Therefore, we investigated the immune functionality of MIF in Chinese mitten crab (Eriocheir sinensis), a commercially important and disease vulnerable aquaculture specie...

Comparative Transcriptome Analysis Reveals Sex-Biased Gene Expression in Juvenile Chinese Mitten Crab Eriocheir sinensis

Sex-biased genes are considered to account for most of phenotypic differences between males and females. In order to explore the sex-biased gene expression in crab, we performed the whole-body transcriptome analysis in male and female juveniles of the Chinese mitten crab Eriocheir sinensis using next-generation sequencing technology. Of the 23,349 annotated unigenes, 148 were identified as sex-...